FOOD RESOURCES 2014. Issue N 4. Technical sciences. Article 09

09. Isolation of the biologically active strains of a sour-milk product of the Caucasus nations
Ts. Korol, D. Naumenko
Pages: 46-52
Georgian matsoni or Armenian matsun – is a traditional fermented milk product, which is referred to a popular the Caucasus drink for longevity, thanks to a unique combination of microorganisms with high biochemical activity. Matsun is produced for a long time, the quality and stability depends on uncontrolled spontaneous fermentation, which can lead to changes in species composition of the product. Therefore, an important issue is to preserve the authenticity of the product by studying the microflora of the "mother" of the leaven and selection of biologically active strains to standardize and improve its quality, without changing the specificity of fermentation. The microflora of the non-commercial fermented milk product matsun have been Investigated. It was found that the dominant lactic acid bacteria groups were genera Lactobacillus, Streptococcus and yeast. General microbiological methods were applied to obtain pure cultures of microorganisms. Allocated 50 isolates with investigational product. Complex morphological and biochemical properties allowed to identificate the following species of lactic acid bacteria: S. thermophilus, L. delbrueckii ssp. bulgaricus, L. delbrueckii ssp. lactis, L. helveticus and E. durans.
Key words: microorganisms, lactic acid bacteria, matsoni, selection, Lactobacillus, Streptococcus, selective medium
2. Uchida K., UrashimaT., Chamnishvili N., Arai I., Motoshima H. Major microbiota of lactic acid bacteria from Matsoni, a traditional Georgian fermented milk // Animal Science Journal. – 2007. – Vol. 78, №1. – P. 85-91.
3. Chanishvili N. Identification of the lactic acid bacterial cultures isolated from the samples of the ca3. ucasian Matsoni // Proc Georgian Acad Sci Biol Ser – 2001. – Vol. 27. – P. 91-95.
4. Soomro A.H., Masud T: Selection of yoghurt starter culture from indigenous isolates of Streptococcus thermophilus and Lactobacillus delbruecki subsp. bulgaricus on the basis of technological properties // Ann Microbiol. 2008. – Vol. 58. – P. 67-71.
5. Quero G.M., Fusco V., Cocconcelli P.S., Owczarek L., Borcakli M., Fontana C., Skapska S., Jasinska U.T., Ozturk T., Morea M. Microbiological, physico-chemical, nutritional and sensory characterization of traditional Matsoni: Selection and use of autochthonous multiple strain cultures to extend its shelf-life // Food Microbiology. – 2014. – Vol. 38, P. 179-191.
6. ГОСТ 10444.11-1989. Продукты пищевые. Методы определения молочно-кислых микроорганизмов. Введ. 1991-01-01. – М.: Изд-во стандартов, 1989. – 13 с.
7. ДСТУ 7357:2013 Молоко та молочні продукти. Методи мікробіологічного контролювання. – Введ. 2014-01-01. – Київ: Мінекономрозвитку України, 2014. 31 с.
8. De Vos P. Bergey’s manual of systematic bacteriology / De Vos P., G.M. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.A. Rainey, K-H. Schleifer, W.D. Whitman. Second edition. Springer: New York, 2009. – Vol. 3. – 1422 p.