FOOD RESOURCES 2019. Issue N 12. Article 07

07BIOTECHNOLOGY AS THE BASIS OF MODERN INNOVATIVE FOOD PRODUCTION TECHNOLOGIES
 
Danylenko S., Naumenko O., Potemska O.
Pages: 64-73
 
Abstract
The main direction of scientific activity of the department of biotechnology is the development of biotechnology of fermentation preparations for the production of fermented products from animal raw materials. Technologies of fermentation preparations provide for obtaining different forms: single- and multicomponent starter cultures, bacterial concentrates, preparations of direct introduction in liquid, dry or frozen state, for the production of sour-milk drinks, hard cheeses, functional products, dried and smoked sausages and forages for animals. The material basis for the technologies of bacterial preparations is the Bank of Microorganism Cultures. Its proper diversity lets it possible to provide advanced technology of new products. With a wide range of industrial strains of different biological properties, it is possible to provide bacterial preparations for both enrichment and fermentation of dairy and meat raw materials. The most promising ones are kept in our own collection and deposited at the State Depository of Industrial Microorganisms of the Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine. Basic fundamental knowledge of the specialists on biotechnology and the profound experience promoted scientific substantiation and development of methodology of production microbiological control at dairy enterprises has been created for Ukraine, which corresponds to the current international practice of the dairy industry and ensures proper control of the quality and safety of products. Detailed methodological approaches were worked out and the basic schemes of implementation of microbiological control of production of various types of dairy products and also, recommendations on methods of microbiological analysis of raw materials, materials, finished products, sanitary-and-hygienic state of production, in accordance with domestic sanitary measures, were developed. Long-term phage monitoring of domestic dairy processing enterprises allowed us to evaluate the overall phage state of production, to obtain pure isolates of industrial phages and highly sensitive strains of microorganisms, and to develop a number of recommendations for manufacturers. In particular, a special test-system for phage monitoring was developed this incorporating strains of lactic acid bacteria with a high sensitivity to different bacteriophages, which can be tailored individually to the specificity of a particular enterprise.
 
Key words: biotechnology, microorganisms, bacteriophages, fermentation cultures, microbiota, probiotic preparations, normoflora, collection
 
References 
1. Novikov V., Sydorov Yu., Shved O. (2008). Tendentsii rozvytku komertsiinoi biotekhnolohii. [Trends in the development of commercial biotechnology]. Visn. NAN Ukrainy. [Bulletin of the National Academy of Sciences of Ukraine]. № 2. Р. 25–39 [in Ukraine].
2. Krasochko P., Krasochko Y., Bolshakov S., Shashkova Yu., Nychik S. (2018). Sostoianie veterynarnoi byotekhnologii v respublyke Belarus na sovremennom etape. [The state of veterinary biotechnology in the Republic of Belarus at the present stage]. Veterynarna biotekhnolohiia [Veterinary biotechnology]. 32 (2). Р. 278–285 [in Russian].
3. Marchuk L. (2013). Biotekhnolohii u konteksti suchasnykh innovatsiinykh zmin. [Biotechnology in the context of modern innovation changes] Visnyk ahrarnoi nauky Prychornomoria. [Ukrainian Black Sea region agrarian science]. V. 2 P. 21–28 [in Ukraine].
4. Berdnikova K. (2016). Analiz suchasnoho stanu ta perspektyvy rozvytku biotekhnolohii v Ukraini. [An analysis of the current state and prospects of biotechnology development in Ukraine]. Materialy KhV Mizhnarodnoi naukovo-praktychnoi konferentsii. Naukovo-tekhnichnyi rozvytok: ekonomika, tekhnolohii, upravlinnia. [Materials of the XV International Scientific and Practical Conference. Scientific and technical development: business, technology, management]. Kiev, P. 25–26 [in Ukraine].
5. Naumenko O., Rozhanska O., Kihel N. Patent na korysnu model № 12788 Ukraina, MPK (2006) S12N1/20; A23S9/12 Shtam bakterii Lactobacillus casei ssp.casei IMB B-7017, shcho vykorystovuietsia u vyrobnytstvi bakterialnykh preparativ ta funktsionalnykh molochnykh produktiv [Strain of bacteria Lactobacillus casei ssp.casei IMB B-7017, used in the manufacture of bacterial preparations and functional dairy products]; Applicant and Patent Attorney Technological Institute of Milk and Meat of the Ukrainian Academy of Agrarian Sciences. № 2004032040; stated. 19.03.04; Bul. 15.03.2006 [in Ukrainian].
6. Kihel N., Taradii H. Patent № 51770 Ukraina, MPK С12N1/20//(С12N1/20, С12R1:23) Shtam bakterii Lactobacillus acidophilus, shcho vykorystovuietsia u vyrobnytstvi zakvashuvalnykh kultur ta funktsionalnykh molochnykh produktiv [The bacterial strain of Lactobacillus acidophilus, used in the production of fermentation crops and functional dairy products] Applicant and Patent Attorney Technological Institute of Milk and Meat of the Ukrainian Academy of Agrarian Sciences. № 99084807; stated. 25.08.1999; Bul. 16.12.2002. [in Ukrainian].
7. Korol Ts., Danylenko S., Kihel N. Patent na korysnu model № 63704 MPK6 C12N1/20, A22S11/00, A23L1/31, A23B4/0 C12R1:25. Shtam bakterii Lactobacillus plantarum, shcho vykorystovuietsia u vyrobnytstvi bakterialnykh preparativ ta fermentovanykh miasnykh produktiv [Strain of bacteria Lactobacillus plantarum, used in the manufacture of bacterial agents and fermented meat products]; Applicant and Patent Attorney Technological Institute of Milk and Meat of the Ukrainian Academy of Agrarian Sciences. № u201004109; stated. 08.04.10; Bul. 25.10.2011. [in Ukrainian].
8. Danylenko S., Kihel N. Patent № 109098 Ukrainy, MPK7 С12N1/2, А61R35/75, A23K1/165. Shtam bakterii Lactobacillus rhamnosus, shcho vykorystovuietsia u vyrobnytstvi funktsionalnykh dobavok dlia silskohospodarskykh tvaryn ta ptytsi [The strain of bacteria Lactobacillus rhamnosus, used in the production of functional additives for farm animals and poultry]. Applicant and Patent Attorney Institute of Food Resources of NAAS. № a20141127; stated. 16.10.14; Bul.. 10.07.2015. [in Ukrainian].
9. Kihel N.F., Nasyrova H.F., Rozhanska O.M., Romanchuk I.O. Patent № 39535 Ukraina, MPK С12N1/20. Sposib oderzhannia bakterialnoho preparatu priamoho vnesennia dlia smetany [A method for obtaining a direct topical bacterial preparation for sour cream]. Applicant and Patent Attorney Technological Institute of Milk and Meat of the Ukrainian Academy of Agrarian Sciences. № 2000105623; stated. 03.10.00; Bul.. 15.06.2001. [in Ukrainian].
10. Yeresko H.O., Potemska O.I., Taradii H.K., Kihel N.F. Patent № 51006 A Ukraina, MPK А23С9/12, С 12N1/20. Sposib oderzhannia bakterialnoho kontsentratu BTP-F dlia vyrobnytstva kyslomolochnykh produktiv [Method of obtaining bacterial concentrate BTP-F for the production of sour-milk products]; Applicant and Patent Attorney Technological Institute of Milk and Meat of the Ukrainian Academy of Agrarian Sciences. № 2001117923; stated. 20.11.01; Bul. 15.11.2002, № 11. [in Ukrainian].
11. Kihel N., Shulha N., Bodnarchuk O. Patent № 74753 Ukraina, MPK (2006) С12N1/20; А23С19/032 (2006.01); А23С9/068 (2006.01) Sposib oderzhannia bakterialnoho kontsentratu priamoho vnesennia „Temp” dlia tverdykh sychuzhnykh syriv z vysokoiu temperaturoiu druhoho nahrivannia [A method of obtaining a direct «Temp» bacterial concentrate for hard cheeses with high temperature of second heating]; Applicant and Patent Attorney Technological Institute of Milk and Meat of the Ukrainian Academy of Agrarian Sciences. № а200503480; stated. 13.04.05; Bul. 16.01.2006. [in Ukrainian].
12. Yeresko H., Naumenko O., Rozhanska O., Ushakova V., Kihel N. Patent № 56385 A Ukraina, MPK A23C9/12, C12N1/20. Sposib oderzhannia bakterialnoho kontsentratu priamoho vnesennia dlia produktiv spetsialnoho pryznachennia [Method for obtaining a direct bacterial concentrate for special purpose products]; Applicant and Patent Attorney Technological Institute of Milk and Meat of the Ukrainian Academy of Agrarian Sciences. № 2001117522; stated. 05.11.01; Bul. 15.05.2003. [in Ukrainian].
13. Danylenko S., Nedorizaniuk L., Semenivska O. Patent № 114255 C2 Ukraina, MPK C12N 1/20 (2016.01), A23B 4/22 (2016.01), C12R 1/25 (2016.01), C12R 1/225 (2016.01), C12R 1/44 (2016.01). Sposib oderzhannia bakterialnoho preparatu “LSt” dlia posolu miasnoi syrovyny [Method for obtaining a bacterial preparation «LST» for the salting of meat raw]. Applicant and Patent Attorney Technological Institute of Milk and Meat of the Ukrainian Academy of Agrarian Sciences. № a2016 01194; stated 12.02.16; Bul. 10.05.2017 [in Ukrainian].
14. Danylenko S., Harda S. Patent № 116253 Ukraina, MPK A23K50 / 00 (2016.01); A23K10 / 16 (2016.01); C12N 1/20 (2006.01Sposib oderzhannia bakterialnoho kontsentratu “BK-Ptytsia” dlia kormovykh produktiv MPK. [Method of obtaining bacterial concentrate «BK-Bird» for fodder products]. Applicant and Patent Attorney Technological Institute of Milk and Meat of the Ukrainian Academy of Agrarian Sciences. № a 2016 01120; stated 10.02.16; Bul. 26.02.2018. [in Ukrainian].
15. Yeresko H., Kihel N., Romanchuk I., Naumenko O., Danylenko S., Orliuk Yu. (2014). Instruktsiia shchodo orhanizatsii vyrobnychoho mikrobiolohichnoho kontroliu na pidpryiemstvakh molochnoi promyslovosti [Instruction on organization of production microbiological control at dairy enterprises]. Kyiv: NNTs «IAE», 372 p. [in Ukrainian].
16. Naumenko O., Kihel N. (2015). Metodychni rekomendatsii z vyznachennia bakteriofahiv na molokopererobnykh pidpryiemstvakh [Methodological recommendations for the determination of bacteriophages in dairy processing enterprises]. Kyiv: NNTs «IAE», 31 p. [in Ukrainian].
17. Danylenko S., Kopіlova E., Verbytskyi S. (2016). Byopreparat dlia эkolohychesky chystoho sylosa [Biopreparat for ecologically pure silage]. Ahro 1. [Agro 1]. № 6. P.8. [in Russian].
18. Danylenko S., Harda S. (2015). Zastosuvannia funktsionalnoi dobavky BK-Pt pry vyroshchuvanni kurchat-broileriv [Application of the functional additive BK-Pt in the cultivation of broiler chickens]. Prodovolchi resursy. [Food Resources]. № 4. P. 117–122. [in Ukrainian].