FOOD RESOURCES 2015. Issue N 5. Article 14

  • Друк
Деталі
Створено: Середа, 16 січня 2019, 09:19
Перегляди: 972
14. Use of selective media to determine species affiliation of lactic acid bacteria
 
Ts. Korol
Pages: 90-98
 
Abstract
Accurate and fast differentiation of mesophilic Lactococcus is an urgent issue, because these organisms are of great industrial importance. The research paper presents nutrient media comparative assessment data (Reddy, Kempler, Agar with calcium acid citrine) for the identification of lactic acid bacteria of the genus Lactococcus, in particular strains Lactoccocus lactis subsp. lactis and Lactoccocus lactis subsp. lactis biovar. diacetylactis. It is shown that taking into account the similarity of the metabolic properties of the strains belonging to the genus Lactococcus the identification procedure should combine application of several selective media. In order to prove belonging to the L. diacetylactis simultaneous cultivation on Kempler and Agar with calcium acid citrine selective media is recommended. It was also featured the expediency of addition of 0.2% sodium citrate to the inoculated medium in order to enhance the flavor-forming properties of L. diacetylactis.
 
Key words:Lactococcus, Lactoccocus lactis subsp. lactis biovar. diacetylactis, selective medium
 
References
1. Özkalp, B. Technological characterization of wild-type Lactococcus lactis strains isolated from raw milk and traditional fermented milk products in Turkey [Text] / B. Özkalp, B. Özden, Y. Tuncer, P. Sanlibaba, M. Akcelik // Lait. – 2007. – Vol. 87, № 6. – P. 521-534.
2. Barrette, J. The production of mixed cultures containing strains of Lactococcus lactis, Leuconostoc cremoris and Lactobacillus rhamnosus, on commercial starter media [Text] / J. Barrette, C.P. Champagne, D. Roy, N. Rodrigue // Journal of Industrial Microbiology & Biotechnology. – 2000. – Vol. 25, № 6. – Р. 288-297.
3. Bergey’s Manual of Systematic Bacteriology: Vol. 3: The Firmicutes [editors: P. De Vos, G.M. Garrity, D. Jones, N.R. Krieg, W.Ludwig, F.A. Rainey, K.-H. Schleifer and W.B. Whitman (Eds.)]. – Second Edition. – USA: Springer-Verlag New York, 2009. – Vol.3 – 1450 p. – ISBN: 978-0-387-95041-9 e-ISBN: 978-0-387-68489-5. – 379 b/w ill., 11 ill. in colour.
4. Nomura, M. Novel characteristic for distinguishing Lactococcus lactis subsp. lactis from subsp. cremoris [Text] / M. Nomura, H.Kimoto, Y. Someya, I. Suzuki // International Journal of Systematic Bacteriology. – 1999. – Vol. 49. – Р. 163-166.
5. Банникова Л.А. Селекция молочнокислых бактерий и их применение в молочной промышленности [Текст] / Л.А.Банникова. – М.: Пищевая пром-сть, 1975. – 256 с.
6. Samaržija D. Taxonomy, physiology and growth of Lactococcus lactis: a review [Text] / D. Samaržija, N. Antunac, J.L.Havranek // Mljekarstvo. – 2001. – Vol. 51, №. 1. – Р. 35-48.
7. Starrenburg Marjo J.C., Hugenholtz J. Citrate Fermentation by Lactococcus and Leuconostoc spp. [Text] / Marjo J.C.Starrenburg, J. Hugenholtz // Appl. Microbiol. Biotechnol. – 1991. – Vol. 57, № 12. – P. 3535-3540.
8. García-Quintáns N. The Citrate Transport System of Lactococcus lactis subsp. lactis biovar diacetylactis Is Induced by Acid Stress [Текст] / Nieves García-Quintáns, Christian Magni, Diego de Mendoza, and Paloma López // Appl. Environ. Microbiol. – 1998, – Vol. 64, № 3. – P. 850-857.
9. Galesloot T.E. Agar media for the isolation and enumeration of aroma bacteria in starters [Text] / T.E. Galesloot, F. Hassing, and J. Stadhouders // Netherlands Milk and Dairy Journal – 1961. –Vol. 15. – P. 127-150.
10. Reddy M.S. Associative Growth Studies in Three-Strain Mixtures of Lactic Streptococcil [Text] / M.S. Reddy, E.R. Vedamuthu, C.J. Washam, G.W. Reinbold // Appl. Microbiol. – 1972. – Vol. 24, No. 6. – P. 953-957.
11. Kempler G.M. Improved medium for detection of citrate-fermenting Streptococcus lactis subsp. diacetilactis [Text] / G.M.Kempler, L.L. McKay // Appl. Environ. Microbiol. – 1980. – Vol. 39, №. 4 – P. 926-927.
12. Банникова Л.А. Микробиологические основы молочного производства / Л.А. Банникова, Н.С. Королева, В.Ф.Семенихина; под ред. канд. техн. наук Я.И. Костина. – М.: Агропромиздат, 1987. – 400 с.: ил.
13. Van Kranenburg R. Flavour formation from amino acids by lactic acid bacteria: predictions from genome sequence analysis [Text] / R. van Kranenburg, M. Kleerebezem, Vlieg J. van Hylckama, B.M. Ursing, J. Boekhorst, B.A.Smit, E.H.E. Ayad, G. Smit, R.J. Siezen // Int. Dairy J. – 2002. – Vol. 12, № 2. – Р. 111-121.
14. Davey G. P. Appearance of the arginine phenotype in Lactococcus lactis subsp. сremoris 2204 following phage transduction [Text] / Graham P. Davey, Howard A. Heap // Canadian Journal of Microbiology. – 1993, Vol. 39, No. 8. – Р. 754-758.
15. ДСТУ 7357:2013 Молоко та молочні продукти. Методи мікробіологічного контролювання [Текст]. – Чинний від 2014-01-01. – Київ: Мінекономрозвитку України, 2014. – III, 35 с.: табл. – (Національний стандарт України).
16. Reddy M.S. Agar Medium for Differential Enumeration of Lactic Streptococci [Text] / M.S. Reddy, E.R. Vedamuthu, C.J.Washam, G.W. Reinbold // Applied Microbiology. – 1972. – Vol. 24, No. 6. – P. 947-952.
17. Залашко М. Определение количества диацетила и ацетоина в закваске и сливочном масле [Text] / М. Залашко, Н.Макарьина // Молочная промышленность. – 1962. – № 10. – С. 42-43.
18. McKay L.L., Baldwin K.A. Applications for biotechnology: present and future improvements in lactic acid bacteria [Text] / McKay L.L., Baldwin K.A. // FEMS Microbiol. Rev. – 1990, Vol. 7, № 1-2. – P.3 -14.
19. Sánchez C. Contribution of Citrate Metabolism to the Growth of Lactococcus lactis CRL264 at Low pH [Text] / Claudia Sánchez, Ana Rute Neves, João Cavalheiro, Margarida Moreira dos Santos, Nieves García-Quintáns, Paloma López and Helena Santos // Appl Environ Microbiol. – 2008. – Vol. 74, № 4 – P. 1136-1144.