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Опубліковано: Понеділок, 23 січня 2023, 10:07
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13. INTENSIFICATION OF THE YEAST GROWING PROCESS BY DISCRETE-IMPULSE PROCESSING OF CULTURAL MEDIA
https://doi.org/10.31073/foodresources2022-19-13
Olexandr Obodovych, Vitalii Sydorenko, Tamila Sheiko
Pages: 116-122
Abstract
Subject of research. Solving the problem of providing yeast cells with a sufficient amount of dissolved oxygen to activate growth and reproduction throughout the entire cultivation period. Purpose. The intensification of the process of aeration of cultural environments by the method of discrete-pulse energy input, implemented in a rotor-pulsation apparatus. Methods. The process of aeration of culture media in the technology of growing Saccharomyces cerevisiae yeast by the method of discrete-pulse energy input was studied. The rate of oxygen absorption in the culture liquid was determined by the amount of yeast biomass grown during the cultivation period. Results. In the course of experiments on the cultivation of yeast on molasses solutions, the dependence of the oxygen mass transfer rate on the angular speed of rotation of the rotor unit in culture media with a dry solid content of 3-10% was determined. With a decrease in the content of solid content from 10 to 5% when processing with an angular speed of 48 rpm, the oxygen absorption rate of the culture liquid increases by 1.9 times. With an increase in the frequency of pulsations from 2 to 3.85 kHz, mass transfer increases from 5 to 7.3 g/l∙h at solid content of 3% and from 2 to 4 g/∙h at solid content of 10%. A further increase in the frequency of pulsations leads to the inactivation of part of the yeast cells. It was also found that the optimal values of the shear rate of the flow is 90-100∙103s-1. Application of the method of discrete-pulse energy input in absorption technologies makes it possible to significantly intensify mass exchange processes. Scope of research results. Oxygen saturation of microorganisms can be used to grow yeast biomass for the bakery and alcohol industries.
Key words: discrete-pulse input of energy, mass transfer, absorption, microorganisms, cultivation, intensification, cultural media

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